The Microbiome of Research Animals - Implications for reproducibility, translatability, and discovery

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Posted in Announcement By Heather Martinez

Microbiome Awards Video

2/6/2018 3:13 PM

 

Meet Dr. Michael Montague, winner of the $65,000 2017 Microbiome Award to study the brain-gut network in free-ranging macaques. Watch the video

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Posted in Culture Dish By Heather Martinez

Molecular Med Tri-Con

2/6/2018 3:03 PM

February 11-16, 2018

San Francisco, CA USA

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Posted in Upcoming Events By Heather Martinez

April 18-20, 2018

Boston, MA USA

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Posted in Upcoming Events By Heather Martinez

 

This webinar will present data on variables capable of influencing the GM of laboratory rodents citing several examples and animal models, considerations related to manipulation of the GM in mice and rats, and recent data supporting the use of “dirty” mice in biomedical research. Register for the webinar

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Posted in Culture Dish Webinar By Heather Martinez

 

As a part of our microbiome interview series, we spoke with Dr. Tasha Santiago-Rodriguez, Scientist and Team Leader Microbiome at the Institute for Life Science Entrepreneurship (ILSE), in New Jersey. Dr. Santiago-Rodriguez received her Ph.D. from the University of Puerto, Rio Piedras. Later, she did her postdoctoral research at University of California, San Diego and California Polytechnic State University. Her research interests include modern and ancient human microbiomes, viromes and resistomes in association with health and disease. We interviewed Dr. Santiago-Rodriguez to discuss her current research and future plans.

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Posted in Announcement Culture Dish By Heather Martinez

 

Breast cancer is the second leading cause of death due to cancer in women worldwide. Breast cancer research has gained worldwide attention and novel approaches have been proposed to combat the disease. This has largely been made possible by generous funding support for breast cancer research and awareness from numerous national and international organizations which has led to improved breast cancer screening, diagnosis and advances in treatment. 

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Posted in Culture Dish By Heather Martinez

 

As a part of our Microbiome Awards winners interview series, we spoke with Dr. Michael Montague, a Postdoctoral Associate in the laboratory of Dr. Michael Platt, University of Pennsylvania in Philadelphia, PA. Dr. Montague received his Ph.D. from New York University. His project aims to study the brain-gut network in free-range macaques to understand the effects of social integration on microbial diversity and serotonin synthesis. He is the grand prize winner of the 2017 Microbiome Awards. We interviewed Dr. Montague to discuss his current research and future plans.

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Posted in Culture Dish By Heather Martinez

 

The gut microbiota composition changes dramatically throughout aging and disease. A healthy gut microbiota is typically characterized by large bacterial taxonomic diversity and functional capacity, whereas frailty and aging are associated with loss of diversity and expansion of more pathogenic bacterial species. However, in order to accurately profile changes in microbial communities, the reproducible isolation of high-quality DNA is an important step. Automation enables reliable and reproducible isolation of DNA of superior quality, which can be used directly for downstream sequencing applications. View the slides

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Posted in Webinar By Heather Martinez

 

This webinar will focus on the automation of QIAGEN’s new line of DNA and RNA sample prep kits for the microbiome. We will show how automation on the QIAcube enables efficient and reliable use of these samples for sensitive downstream applications such as qPCR and NGS. In addition, you will learn how to successfully use the CLC Microbial Genomics Module for metagenome sequencing and identification of microbial composition and diversity. View the slides

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Posted in Webinar By Heather Martinez

 

The journey of combating bacteremia starts with the isolation of bacterial DNA from infected blood. At QIAGEN, we have an innovative solution, the QIAamp BiOstic Bacteremia DNA Kit. When the QIAamp BiOstic Bacteremia DNA Kit was originally designed, it was optimized for the extraction of bacterial DNA from infected blood. However, it has turned out to be useful for so much more.

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Posted in Culture Dish Technical Tips By Heather Martinez

 

As a part of our Microbiome Awards winners interview series, we spoke with Dr. Andrea M. Tarnecki, a Postdoctoral Research Fellow at Mote Marine Laboratory in Sarasota, Florida. Dr. Tarnecki received her Ph.D. from Auburn University, and her research interests include probiotics for use in aquaculture and deciphering beneficial host-microbe interactions. She is a recipient of the 2016 Microbiome Award. 

 

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Posted in Culture Dish By Heather Martinez

 

This webinar will focus on the acquisition and development of the preterm gut microbiome from birth and following discharge from intensive care. Specifically, we will discuss the association of the gut microbiome with necrotizing enterocolitis (NEC) and late onset sepsis (LOS), as well as the impact of birth mode. We will also discuss analysis of multi-omic datasets, including the analysis of the airway microbiome and metabolome in infants hospitalized with bronchiolitis. View the slides

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Posted in Webinar By Heather Martinez

 

Christopher Stewart is a Postdoctoral Associate at Baylor College of Medicine in Houston, Texas. He completed his Ph.D. at Northumbria University Newcastle upon Tyne in 2014 and later moved to the United States for a postdoctoral fellowship. His research interests include the diversity and ecology of microbial communities and host responses in clinical samples and determining the microbial-host interaction in health and disease. He was one of the winners of the 2015 Microbiome Awards. We interviewed Dr. Stewart to discuss characterization of the microbiome of neonates and infants in relation to health and disease.

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Posted in Culture Dish By Heather Martinez

 

Microbiomes, localized communities of microorganisms that exist symbiotically with their immediate environment, can be found virtually anywhere; inside the human colon, around plant roots, inside coral reefs and even within ant colonies. These little microbial microcosms are a hot topic right now. Recent studies have implicated their role in the wellbeing of people, animals, plants and entire oceans (1–3). 

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Posted in Culture Dish By Heather Martinez

 

In this webinar, we will introduce the latest workflows optimized for shotgun metagenome analysis, which we released with the most recent version of Microbial Genomics Pro Suite – our comprehensive, user-friendly and scalable NGS data analysis platform for microbial genomics. View the recording

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Posted in Webinar By Heather Martinez

 

As part of our research on the best practices for soil microbial DNA extraction, we collected a wide variety of samples for product development. Therefore, when we were developing the protocols for the PowerLyzer 24 Homogenizer, we developed a protocol that is applicable for the majority of the samples tested. 

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Posted in Culture Dish Technical Tips By Heather Martinez

 

In a recent study, Willmann and colleagues investigated the impact of ciprofloxacin treatment on the intestinal microbiome. Antimicrobial treatments are known to upset the structure of the intestinal microbiome. Taxonomic profiling can reveal the severity and long term effects of antibiotic treatments on the gut community.

 

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Posted in Culture Dish By Brittanie Collinsworth

 

QIAGEN receives so many questions about DNA and RNA isolation from stool! One of the most frequently asked questions is how to store fecal samples for shipping and for further processing. 

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Posted in Culture Dish Technical Tips By Heather Martinez

Cleaning up dirty DNA

3/13/2017 1:23 PM

 

QIAGEN is cleaning up… DNA and RNA that is. With the addition of the DNeasy and RNeasy PowerClean Pro Cleanup Kits to our portfolio, we thought it would be a great time to discuss how cleanup kits work and when it is appropriate to use one. 

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Posted in Culture Dish Technical Tips By Heather Martinez

 

In this webinar, we introduce the scientist-friendly Microbial Genomics Pro Suite offering workflows optimized for microbiome profiling, microbial typing and outbreak analysis. The workflows and tools for microbial genomics introduced with this software package are further extending the comprehensive set of genomics, transcriptomics and epigenomics analysis solutions that researchers know from CLC Genomics Workbench. View the slides

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Posted in Webinar By Heather Martinez

 

In this webinar, learn about the innovative technologies that form the basis of QIAGEN’s portfolio of QIAseq library prep solutions for metagenomics and microbiome sequencing. Whether your research starts from single microbial cells, 16s rRNA PCR amplicons, or gDNA for whole genome analysis, QIAseq technologies offer tips and tricks for capturing the genomic diversity of your samples in the most unbiased, streamlined way possible. View the slides

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Posted in Webinar By Heather Martinez

 

Gut homeostasis is regulated by complex interactions between genetic and environmental factors and the host’s immune system. When this homeostasis is disrupted, gut inflammation ensues. Microbial imbalance, also known as dysbiosis, plays a critical role in intestinal pathology; however, previous studies have only focused on commensal bacteria despite metagenomic studies showing that a variety of resident viruses also inhabit the healthy gut.

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Posted in Culture Dish By Heather Martinez

 

An accurate molecular analysis of the microbial constituents of a particular community is contingent upon high-yielding and non-biased nucleic acid extraction methodologies. Only by ensuring that all species and classes of microorganisms present in a sample are effectively lysed during extraction will one be able to reliably assess the composition of that sample. An additional challenge faced in nucleic acid extraction is the presence of persistent, co-purifying polymerase inhibitors endogenous to one’s sample. View the slides

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Posted in Webinar By Heather Martinez