This weeks technical tip is a great question because, as we are all aware, no two environmental samples are ever quite alike. And when working with microbes, they exist anywhere and everywhere, so the substrate often complicates the matter. That's why choosing a method for DNA isolation can be confusing. Luckily, here at MO BIO, we've seen and heard it all and we know what to do.
Take this question, for example.....
Hi MO BIO Technical Support,
I'm considering using one of your PowerSoil kits for an experiment I'm doing. I'm not actually extracting from soil, however. I'm extracting fungal DNA from wood and paper that I've allowed to be colonized by fungi in the environment.
It sounds like combining the Powersoil kit with the Powerlyzer bead-beating would take care of fungal cell disruption and humics from the wood & paper.
However, I'm concerned about polysaccharides and polyphenolics, which may potentially be present in high concentrations in these samples. Would "inhibitor removal technology" be able to remove these inhibitors? Could you fill me in as to whether this kit would be good to use on substrates that might contain high amounts of polysaccharides and polyphenolics? If so, are any changes to the protocol provided for soil necessary?
Thanks for your help.Read More