DNA so Clean, it's Obscene

11/13/2013 5:41 PM

MO BIO is cleaning up...DNA & RNA that is. With the release of our new PowerClean Pro kits, we thought it would be a great time to discuss how clean-up kits work and when you'd want to use one. Clean-up kits take dirty nucleic acids and remove contaminants that could interfere with your downstream business. Enzyme-dependent applications like restriction digests, ligations, PCR amplification and sequencing all require squeaky clean DNA & RNA in order to get the best (or sometimes any!) results. Choosing the right clean-up kit for your needs depends on which substances you want to remove. Not sure? It depends on the source. Typically these reaction-killing interlopers are left over from either a previous enzymatic reaction or from the organism/environment that the DNA/RNA was extracted from. Our clean-up kits can be divided into two types based on which of these is the source of contamination.

Read More
Posted in Technical Tips By Michelle Tetreault Carlson

How Dirty is DNA Without IRT?

5/23/2012 12:00 AM

Scientists often come to us with their dirty little DNA problems.   Samples like soil, feces, and blood (oh my!) can make extracting DNA challenging because they are high in compounds like humic acids, polysaccharides, heme, or dyes.  These bind to the DNA and inhibit enzymes used in downstream applications like PCR and sequencing.   MO BIO uses patented Inhibitor Removal Technology® (IRT), a method to remove these substances in many of our kits.   It is very effective at removing the inhibitory compounds without significantly decreasing DNA yield.   But how well does it really work?   Good scientists want to see the data. Recently we received the following request...

Read More
Posted in Technical Tips By Michelle Tetreault Carlson

Welcome back readers! After a short break from blogging, we're back with some new and different ideas to share with you. We'll start off with a new weekly feature called Tech Tip Thursday (#MBTTT on twitter) where we share some of our customer's questions and our answers with you. What better way of saving you time for life then to post a few of the frequently asked questions we get from all the scientists out there using our products and our answers to those questions? We figured you'd agree.

 

This week I'd like to share a question about the storage of environmental samples in ethanol as a preservative and why this is NOT a good idea. 

Read More
Posted in Technical Tips By Suzanne Kennedy