Today I wanted to talk about a method that you’ve been doing forever in your science career. Something so basic, so easy, that I bet you don’t even have to think about it. I bet you can do this type of prep in your sleep. What am I talking about? Plasmid preps, of course!
Plasmid DNA isolation is so routine today in labs that you pretty much expect to get DNA back, even when you make a mistake. But are you getting back only DNA? It turns out that plasmid preps are the perfect application to demonstrate a basic difference in two methods for DNA quantification: spectrophotometry (Nanodrop) vs. fluorescent dye (Picogreen).Read More