- Service Description
- Experienced - MO BIO has over 22 years of experience in providing RNase-free certifications to many major medical device companies and suppliers of plastic consumables
- Qualified - All MO BIO Services technicians receive extensive training and are qualified to test raw materials or end products for the presence of RNase
A ribonuclease, or RNase, is an enzyme which degrades RNA into smaller nucleotides. Sources of RNase contamination in the laboratory include human skin, air, and dust. RNA is extremely susceptible to degradation, so RNase contamination is of great concern in the medical device and pharmaceutical industries as well as the biotech and research fields. RNase can cause degradation of valuable RNA samples, which may make it impossible to analyze the RNA via RT-PCR, RT-QPCR or RNA sequencing.
Certifying products RNase-free has become a common practice among general laboratory plastics manufacturers as well as medical device and pharmaceutical companies. Testing your product lines for this common contaminant will add integrity to your products and will enable a wider range of customers to utilize your products for their experiments and applications.
RNase Test Method
All testing apparatus are treated with Diethyl Pyrocarbonate (DEPC) to inhibit possible RNase contamination of the experiment from outside sources. Test samples of the product are extracted and a portion of extract is incubated with an RNA standard (6 kb RNA) in a buffered solution with both sodium and magnesium ions. Negative and Positive controls are run with the test sample extract to validate the experimental procedure and technique. An unexposed RNA standard is included to represent the negative control. An RNA standard exposed to RNase is also included to represent the positive control.
All samples are incubated 1 hour at 37°C. After the incubation period, the samples and controls are evaluated by agarose gel electrophoresis and a picture for the Certificate of Analysis and final report is taken. The test samples and the negative control should show no degradation. The positive control should be a very blurry, quick-running band, indicating the effect of RNase contamination on the RNA standard pool that is used for all the samples.
Passing test criteria: The RNA exposed to the test sample or extract must look exactly the same as the RNA negative control that was exposed to sterile water. The negative control must be completely intact with no degradation. The positive control must show degradation of the RNA standard for the test to be valid.
Test Sensitivity: 10-9 Kunitz Units/ µl.
How To Begin Certifying Your Products RNase-Free
To begin certifying your product RNase Free, a sample of the product must be received by our Services Division to evaluate how best to extract your particular product for testing. Once an extraction procedure is approved, it is used each time that product is received for testing. Many products will fit one of our pre-designed extraction procedures, such as microcentrifuge tubes, PCR tubes, pipet tips and PCR plates. However, not all products are the same, thus all products are evaluated on an individual basis. Liquid solutions and reagents as well as solids, due to the diversity of these products, require a separate test procedure to be designed each time a product of this type is submitted.
- Contact Services
Our Services team has over 23 years of experience in providing services that keep your products competitive and in demand. Our customers include many of the top plastics and medical device suppliers in the industry. Our services specialists can also work with you to develop a custom testing schedule and/or custom tests that fit the specific needs of your products. We are located in Carlsbad, CA, USA.