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DNeasy PowerWater Sterivex Kit

Isolate genomic DNA from water samples collected with Sterivex Filter Units.
  • Fast - 40 minute protocol from start to finish
  • In-unit digestion - No need to struggle with removing filters from Sterivex Units
  • No harsh chemicals - Column-based purification protocol eliminates the need for phenol/chloroform extraction
The DNeasy PowerWater Sterivex Kit is the only kit available to isolate genomic DNA from Millipore's Sterivex filter units without the need for enzymes or hazardous organic chemicals. Utilizing a novel Cell Release Solution, microbes are released from the Sterivex filter units without extensive incubation times or having to cut open the plastic casing holding the membrane, enabling purification of high quality DNA in just 40 minutes. Patented Inhibitor Removal Technology® is included to provide high quality DNA from all types of water samples, even those containing heavy amounts of contaminants. In addition, a unique silica binding column and column extender enable one-step addition of a large volume of solution (4.5 ml) and elution in a 50-100 µl volume.

The DNeasy PowerWater Sterivex Kit protocol starts with the addition and incubation of the Sterivex units with Cell Release Solution. Lysis buffer is then added to the units, followed by mixing and removal of the lysate for homogenization in a 5 ml bead beating tube. After the protein and inhibitor removal steps, total genomic DNA is captured on a silica binding column using a vacuum manifold. High quality DNA is then washed and eluted from the binding column membrane for use in downstream applications including PCR, qPCR, Sanger sequencing and NGS. This kit is recommended for use with Millipore catalog# SVGPL10RC Sterivex GP Filter units, but is also compatible with other Sterivex Filter units.

Formerly known as the PowerWater Sterivex DNA Isolation Kit.
Cat No./ID: 14600-50-NF
DNeasy PowerWater Sterivex Kit (50)
Isolate genomic DNA from water samples collected with Sterivex Filter Units.
The DNeasy PowerWater Sterivex Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.