AllPrep Bacterial DNA/RNA/Protein Kit
- Powerful lysis - Optimized bead beating method enables efficient lysis and solubilization of total nucleic acids and cellular proteins from gram-negative and gram-positive bacteria
- Easy and fast protein extraction - Novel spin filter-based method enables sequential DNA, RNA and protein extraction in less than 40 minutes without the need for precipitation
- Accurate results – Nucleic acids can be used in PCR, qPCR, RT-PCR, and next-generation sequencing; protein can be used for mass spectrometry, 1D and 2D gel electrophoresis
This novel sequential isolation method begins with mechanical lysis of cultured bacterial cells using a 0.1 mm glass bead tube. Nucleic acids and proteins are completely solubilized during homogenization and mixed with a DNA binding solution. DNA is bound to a spin column and the flow-through containing RNA and proteins is then combined with a solution that binds total RNA on a second spin column. The final flow-through, containing denatured proteins, is combined with another novel buffer to immobilize the proteins onto the third and final silica spin column. Each spin column containing either immobilized nucleic acids or proteins is then washed and the immobilized analyte is eluted.
For isolation of DNA, RNA and Protein from Fungal cultures, try our AllPrep Fungal DNA/RNA/Protein Kit.
Cat No./ID: 47054
AllPrep Bacterial DNA/RNA/Protein Kit (50)
Isolate total nucleic acids and cellular proteins from bacterial cultures.
The AllPrep Bacterial DNA/RNA/Protein Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
fragment fix placeholder