DNase Max Kit
- High activity, stable DNase I – Room temperature stable enzyme does not require freeze thaws and maintains the highest activity
- Protects RNA – Certified RNase free, no heat, EDTA or harsh chemicals required
- DNase Removal Resin – Completely removes DNase and divalent cations for optimal qPCR results
- Fast – 30 minute protocol from start to finish
Preserve enzyme activity
DNase Max Enzyme a high activity DNase I enzyme which is stable at room temperature, so there is no need to aliquot and freeze stocks of the enzyme. Room temperature stability eliminates concern about freeze-thaw cycles that may decrease enzymatic activity. DNase Max Enzyme maintains the highest activity over the life of the kit, enabling consistent results with all RNA samples.
Protect your RNA
Isolating high quality RNA is a time-consuming and expensive process. Consequently, it is essential to prevent RNA degradation during DNase treatment. The DNase Max Kit protects valuable RNA samples during this process, as it contains only Certified RNase-free reagents, and does not require heat, EDTA or harsh chemicals.
The DNase Max Kit protocol starts with dilution of DNase Max Enzyme and 10X DNase Max Buffer to a final concentration of 1X in the digestion reaction. The reaction is then incubated at 37°C for 20 minutes. Removal of the DNase and divalent cations is performed by adding DNase Max Removal Resin and incubating for 10 minutes at room temperature. The resin is pelleted by centrifugation, and the RNA sample is transferred to a new tube, ready for use in RT-PCR and further analysis.