DNeasy PowerSoil Kit

DNeasy PowerSoil Kit

Isolate microbial genomic DNA from all soil types.

Size/Component Catalog #
50 preps 12888-50
100 preps 12888-100
Product Description

Details

  • Optimized lysis for tough samples – Isolate pure DNA from soil, stool, and other environmental samples
  • Inhibitor Removal Technology - Eliminates humic substances and other PCR inhibitors, for DNA that is ready to use in PCR, qPCR, Sanger sequencing and NGS
  • Rapid protocol - Enables isolation of high quality, pure DNA from 250 mg samples in just 30 minutes

The DNeasy PowerSoil Kit uses patented Inhibitor Removal Technology to isolate microbial genomic DNA from all soil types and environmental samples, as well as fecal, stool and biosolid samples. The isolated DNA has the highest level of purity, enabling successful PCR and qPCR amplification and Sanger sequencing or NGS of organisms from the sample. The kit is ideal for processing all environmental samples including those containing high humic acid content such as compost, sediment, and manure. PCR analysis has been performed to detect a variety of organisms including both Gram-positive and Gram-negative bacteria (e.g. Bacillus subtilis, Bacillus anthracis), fungi (e.g. yeasts, molds), algae, and actinomycetes (e.g. Streptomyces), and nematodes. 

Formerly known as PowerSoil DNA Isolation Kit.

Specs

Specs

Format Silica Spin Filter Tubes
Method Bead Beating
Sample Size 0.25 g
Binding Capacity Up to 20 µg per filter
Throughput 1-24 samples
Time 30 minutes
Storage Store at room temperature (15-30°C)
Bead Type 0.7 mm garnet
Sample Types Processed Soil, fecal, water, food, insects, swabs with PCR inhibitors
Equipment Required Vortex and Vortex Adapter (13000-V1-24)
Catalog # 12888-50, 12888-100, 12888-S
Old Name PowerSoil DNA Isolation Kit
SDS/Protocol

SDS/Protocol

Protocol

SDS

Components
Product name Price
DNeasy PowerSoil Kit Components - 100 preps
$16.00
Data

Data

High Yield, Intact Genomic DNA 
  
DNeasy PowerSoil
Supplier A
Supplier B
  
Total Genomic DNA was isolated from nine different sample types using the DNeasy PowerSoil Kit and soil DNA isolation kits from two other suppliers. All isolations were performed following the manufacturer's protocols. Eluted genomic DNA was displayed on a 0.8% TAE agarose gel (15 µl per lane).
  
Confidence in Your PCR Analysis 
  
DNeasy PowerSoil   
Supplier A
Supplier B
  
PCR analysis using eubacterial primers was performed on 1 µl of the undiluted DNA elute. PCR products were displayed on a 0.8% TAE agarose gel. 
  
Lane 1 - Marker   Lane 6 - Marine Sediment
Lane 2 - Landfill 0-3 inches   Lane 7 - Lake Sediment
Lane 3 - Landfill 3-6 inches   Lane 8 - Mud Sediment
Lane 4 - Late-stage Compost   Lane 9 - Horse Manure
Lane 5 - Coffee Compost   Lane 10 - Mulch Topsoil
     

Only DNA isolated with DNeasy PowerSoil yielded a PCR product for Landfill samples (lanes 1 and 2), Lake Sediment (lane 6) and Mulch Topsoil (lane 9). DNA isolated using other supplier kits failed to amplify products for these samples, most likely due to high levels of humic acid substances remaining in the final DNA eluate. 

Positive PCR amplification using DNeasy PowerSoil was observed with 100% of the samples. PCR analysis resulted in only 44% positive amplification with other supplier kits tested.

Publications

Publications

Note: the DNeasy PowerSoil Kit was formerly known as the PowerSoil DNA Isolation Kit.