QIAamp BiOstic Bacteremia DNA Kit

QIAamp BiOstic Bacteremia DNA Kit
Isolate bacterial DNA from cultured blood, fecal and cervical swabs.
Size/Component Catalog #
50 preps 12240-50
Product Description

Details

  • Rigorous lysis method - Ensures complete lysis of tough organisms and high yield of microbial DNA even from blood samples with low bacterial cell counts
  • Inhibitor Removal Technology - Eliminates inhibitors from blood and culture medium for robust amplification of bacterial DNA
  • Fast protocol - isolate DNA in less than 45 minutes

The QIAamp BiOstic Bacteremia DNA Kit enables efficient purification of bacterial DNA from cultured blood, fecal and cervical swabs while completely removing PCR inhibitors common in blood, swabs, and culture medium. Bacterial genotyping from cultured blood can be difficult due to the presence of PCR inhibitors such as charcoal used for the removal of antibiotics from patient blood (e.g. BacT/ALERT tubes).The QIAamp BiOstic Bacteremia DNA Kit utilizes patented Inhibitor Removal Technology combined with mechanical bead beating lysis to isolate pure genomic DNA that is free of inhibitory substances and ready to use in bacterial genotyping assays such as the Bacterial Barcodes Rep-PCR assays and other multiplex PCR or real-time PCR assays, as well as Sanger sequencing and NGS.The QIAamp BiOstic Bacteremia DNA Kit has been successfully tested on gram - and gram + bacteria.

Formerly known as the BiOstic Bacteremia DNA Isolation Kit.

Specs

Specs

Format Silica Spin Filter Tubes
Method Bead Beating
Sample Size 1.8 ml of cultured blood
Binding Capacity Up to 20 µg per filter
Throughput 1-24 samples
Time 45 minutes
Storage Store at room temperature (15-30°C)
Bead Type 0.15 mm garnet
Sample Types Processed Cultured blood, swab samples which contain PCR inhibitors (fecal, vaginal, wound, intestinal/stomach, environmental surfaces), microbial pellets containing PCR inhibitors, uncultured blood, filtered plant washes, feces, raw milk
Equipment Required Vortex
Catalog # 12240-50, 12240-S
Old Name BiOstic Bacteremia DNA Isolation Kit
SDS/Protocol
Publications

Publications

Formerly known as the BiOstic Bacteremia DNA Isolation Kit.