DNeasy PowerFood Microbial Kit

DNeasy PowerFood Microbial Kit
Isolate inhibitor-free DNA from a variety of cultured foods.
Size/Component Catalog #
100 preps - Including 0.5 ml bead tubes 21000-100
100 preps - Including 2 ml bead tubes 21000-100-MON
Product Description

Details

  • Optimized lysis for tough microbes - Enables isolation of high-quality DNA from a variety of food types and pathogens (gram - and gram +), yeasts, and fungi
  • Inhibitor Removal Technology - Eliminates PCR inhibitors, including lipids and polysaccharides, for DNA that is ready to use in PCR, qPCR, genotyping, Sanger sequencing or NGS
  • Streamlined, easy-to-use protocol - Makes training technical staff easier

The DNeasy PowerFood Microbial Kit is designed to isolate high-quality genomic DNA from food cultured microorganisms. The DNeasy PowerFood Microbial Kit stands out from other commercial food kits with its easy-to-use protocol that enables isolation of DNA from a variety of food pathogens. Further, it is the only complete kit on the market, eliminating the need to purchase additional reagents and enzymes.The DNeasy PowerFood Microbial Kit incorporates our patented Inhibitor Removal Technology to remove PCR-inhibiting compounds associated with food cultures, including lipids and polysaccharides. The kit’s buffers provide high quality, inhibitor-free DNA for use in downstream applications including PCR, qPCR and genotyping. Food samples examined using the DNeasy PowerFood Microbial Kit include meats, cheeses and dairy products, chocolate, fruits, vegetables, and juices. Food pathogen DNA isolated from the following organisms was shown to be PCR inhibitor free: Clostridium difficile, Clostridium perfringens, Enterococcus faecalis, Escherichia coli, Lactobacillus sp., Listeria monocytogenes, and Salmonella enterica.

The DNeasy PowerFood Microbial Kit is available with either 0.5 ml bead tubes containing 0.15 mm garnet beads (21000-100), or with 2 ml bead tubes containing 0.15 mm garnet beads (21000-100-MON). The 0.5 ml bead tubes are best for DNA isolation from food cultures, while the 2 ml bead tubes work best for isolation of DNA from uncultured food. Please note, 21000-100-MON requires a minimum order of 6 kits.

Formerly known as the PowerFood MIcrobial DNA Isolation Kit.

Specs

Specs

Format Silica Spin Filter Tubes
Method Bead Beating
Sample Size 1.8 ml cultured food or 0.25 g of food material
Binding Capacity Up to 20 µg per filter
Throughput 1-24 samples
Time 25 minutes
Storage Store at room temperature (15-30°C)
Bead Type 0.15 mm garnet
Sample Types Processed Cultured foods, dairy (milk, yogurt, cheese), juices, vegetables, fruits, chocolate, washes
Equipment Required Vortex and Vortex Adapter (13000-V1-24)
Catalog # 21000-100, 21000-100-MON, 21000-S
Old Name PowerFood Microbial DNA Isolation Kit

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SDS/Protocol
Publications

Publications

Formerly known as the PowerFood Microbial DNA Isolation Kit.