DNeasy PowerSoil Kit

• Optimized lysis for tough samples – Isolate pure DNA from soil, stool, and other environmental samples
• Inhibitor Removal Technology - Eliminates humic substances and other PCR inhibitors, for DNA that is ready to use in PCR, qPCR, Sanger sequencing and NGS
• Rapid protocol - Enables isolation of high quality, pure DNA from 250 mg samples in just 30 minutes

The DNeasy PowerSoil Kit uses patented Inhibitor Removal Technology to isolate microbial genomic DNA from all soil types and environmental samples, as well as fecal, stool and biosolid samples. The isolated DNA has the highest level of purity, enabling successful PCR and qPCR amplification and Sanger sequencing or NGS of organisms from the sample. The kit is ideal for processing all environmental samples including those containing high humic acid content such as compost, sediment, and manure. PCR analysis has been performed to detect a variety of organisms including both Gram-positive and Gram-negative bacteria (e.g. Bacillus subtilis, Bacillus anthracis), fungi (e.g. yeasts, molds), algae, and actinomycetes (e.g. Streptomyces), and nematodes. 

Formerly known as PowerSoil DNA Isolation Kit.

Protocol

• 12888

SDS

• 12888-50
• 12888-100

High Yield, Intact Genomic DNA
DNeasy PowerSoil
Supplier A
Supplier B
Total Genomic DNA was isolated from nine different sample types using the DNeasy PowerSoil Kit and soil DNA isolation kits from two other suppliers. All isolations were performed following the manufacturer's protocols. Eluted genomic DNA was displayed on a 0.8% TAE agarose gel (15 µl per lane).
Confidence in Your PCR Analysis
DNeasy PowerSoil
Supplier A
Supplier B
PCR analysis using eubacterial primers was performed on 1 µl of the undiluted DNA elute. PCR products were displayed on a 0.8% TAE agarose gel.

Only DNA isolated with DNeasy PowerSoil yielded a PCR product for Landfill samples (lanes 1 and 2), Lake Sediment (lane 6) and Mulch Topsoil (lane 9). DNA isolated using other supplier kits failed to amplify products for these samples, most likely due to high levels of humic acid substances remaining in the final DNA eluate. 

Positive PCR amplification using DNeasy PowerSoil was observed with 100% of the samples. PCR analysis resulted in only 44% positive amplification with other supplier kits tested.

Note: the DNeasy PowerSoil Kit was formerly known as the PowerSoil DNA Isolation Kit.

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