DNeasy PowerSoil HTP 96 Kit

DNeasy PowerSoil HTP 96 Kit
For high-throughput isolation of DNA from up to 384 soil samples in less than one day.
Size/Component Catalog #
4 x 96 Preps 12955-4
Product Description

Details

  • Optimized lysis for tough samples – Isolate pure DNA from soil, stool, and other environmental samples
  • Inhibitor Removal Technology - Eliminates humic substances and other PCR inhibitors, for DNA that is ready to use in PCR, qPCR, Sanger sequencing or NGS
  • High-throughput method - Isolate high quality, pure DNA from 250 mg samples in a convenient 96 well format

The DNeasy PowerSoil HTP 96 Kit is comprised of a novel and proprietary method for high-throughput isolation of genomic DNA from environmental samples in a 96 well format. The kit is intended for use with soil and other environmental samples including difficult soil types such as compost, marine sediment, and manure. The isolated DNA has a high level of purity allowing for more successful PCR amplification of DNA from organisms within the sample. PCR analysis has been performed to detect a variety of organisms including both Gram-positive and Gram-negative bacteria (e.g. Bacillus subtilis, Bacillus anthracis), fungi (e.g. yeasts, molds), algae, actinomycetes (e.g. Streptomyces) and nematodes. Environmental samples are added to a 96 well bead beating plate for rapid and thorough homogenization. Cell lysis occurs by mechanical and chemical methods. Total genomic DNA is captured on a 96 well silica membrane in a spin column format. DNA is then washed and eluted from the spin column. DNA is ready for PCR, qPCR or next generation sequencing.

Formerly known as the PowerSoil-htp 96 Well Soil DNA Isolation Kit

Specs

Specs

Format Silica Spin Filter Plates
Method Bead Beating
Sample Size 0.25 g
Binding Capacity Up to 20 µg per filter
Throughput 96-384 samples
Time Process 384 samples in less than one day
Storage Store at room temperature (15-30°C)
Bead Type 0.7 mm garnet
Sample Types Processed Soil, fecal, water, food, insects, swabs with PCR inhibitors
Equipment Required TissueLyser II and Plate Adapter Set
Catalog # 12955-4
Old Name PowerSoil-htp 96 Well Soil DNA Isolation Kit
SDS/Protocol

SDS/Protocol

Protocol

SDS

Components
Product name Price
DNeasy PowerSoil HTP 96 Kit Components
$38.00
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Publications

Publications

Formerly known as the PowerSoil-htp 96 Well Soil DNA Isolation Kit.