- Product Description
- Rapid, high-throughput protocol - Process 96 microbial samples in 1.5 hours
- Optimized lysis for tough microbes - Bead-based homogenization method enables isolation of DNA from yeast, fungi, Gram-negative and Gram-positive bacteria
- High quality DNA - Ready to use in PCR, restriction digests, Sanger sequencing and NGS
The DNeasy UltraClean 96 Microbial Kit is designed to isolate high quality genomic DNA from microorganisms in a 96 well format. A variety of microorganisms including Gram-negative and Gram-positive bacteria, yeast, and bacterial and fungal spores have been tested successfully with this kit. The microbial cells are added to a bead beating plate containing beads, bead solution and lysis solution. The principal is to lyse the microorganisms by a combination of heat, detergent, and mechanical force using specialized beads. The cellular components are lysed by mechanical action using a TissueLyser II. The released DNA is bound to a silica spin plate. The plate is washed, and the DNA is recovered in certified DNA-free Tris buffer.
Format Silica Spin Filter Plates Method Bead Beating Sample Size 1.8 ml Binding Capacity Up to 20 µg per filter Throughput 96 samples Time 1.5 hours Storage Store at room temperature (15-30°C) Bead Type 0.1 mm glass Sample Types Processed Cultured gram-negative bacteria, gram-positive bacteria, yeast, fungi Equipment Required TissueLyser II and Plate Adapter Set
Centrifuge with 96 well plate swinging bucket rotor at least 5.5 cm deep
Catalog # 10196-4 Old Name UltraClean® -htp 96 Well Microbial DNA Kit
Consistent yields of high quality DNA from various microorganisms Lane 1: Marker Lane 2: E. coli Lane 3: E. faecalis Lane 4: B. subtilis Lane 5: P. fluorescens Lane 6: C. albicans Lane 7: C. albicans Lane 8: S. cerevisiae Lane 9: S. cerevisiae DNA was purified from various microorganisms and displayed on 1.2% TAE agarose gel.
- Zhang, L., Srinivasan, U., Marrs, C., Ghosh, D., Gilsdorf, J., & Foxman, B. (2004). Library on a slide for bacterial comparative genomics. BMC Microbiology, 4(1), 12. doi:10.1186/1471-2180-4-12