DNeasy UltraClean 96 Microbial Kit

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DNeasy UltraClean 96 Microbial Kit
For high-throughput isolation of DNA from microbial cultures.
Size/Component Catalog #
4 x 96 Preps 10196-4
Product Description

Details

  • Rapid, high-throughput protocol - Process 96 microbial samples in 1.5 hours
  • Optimized lysis for tough microbes - Bead-based homogenization method enables isolation of DNA from yeast, fungi, Gram-negative and Gram-positive bacteria 
  • High quality DNA - Ready to use in PCR, restriction digests, Sanger sequencing and NGS

The DNeasy UltraClean 96 Microbial Kit  is designed to isolate high quality genomic DNA from microorganisms in a 96 well format. A variety of microorganisms including Gram-negative and Gram-positive bacteria, yeast, and bacterial and fungal spores have been tested successfully with this kit. The microbial cells are added to a bead beating plate containing beads, bead solution and lysis solution. The principal is to lyse the microorganisms by a combination of heat, detergent, and mechanical force using specialized beads. The cellular components are lysed by mechanical action using a TissueLyser II. The released DNA is bound to a silica spin plate. The plate is washed, and the DNA is recovered in certified DNA-free Tris buffer. 

Specs

Specs

Format Silica Spin Filter Plates
Method Bead Beating
Sample Size 1.8 ml
Binding Capacity Up to 20 µg per filter
Throughput 96 samples
Time 1.5 hours
Storage Store at room temperature (15-30°C)
Bead Type 0.1 mm glass
Sample Types Processed Cultured gram-negative bacteria, gram-positive bacteria, yeast, fungi
Equipment Required TissueLyser II and Plate Adapter Set
Centrifuge with 96 well plate swinging bucket rotor at least 5.5 cm deep
Catalog # 10196-4
Old Name UltraClean® -htp 96 Well Microbial DNA Kit
SDS/Protocol

SDS/Protocol

Protocol

SDS

Data

Data

Consistent yields of high quality DNA from various microorganisms
 Consistent DNA Yields with UltraClean®-htp 96 Well Microbial DNA Isolation Kit
 
Lane 1: Marker
Lane 2: E. coli
Lane 3: E. faecalis
Lane 4: B. subtilis
Lane 5: P. fluorescens
Lane 6: C. albicans
Lane 7: C. albicans
Lane 8: S. cerevisiae
Lane 9: S. cerevisiae
 
DNA was purified from various microorganisms and displayed on 1.2% TAE agarose gel.
Publications

Publications

Documents

Documents

“DNA