- Product Description
- High-throughput method - Isolate high quality, pure DNA from plant samples in a 96 well format
- Optimized for tough samples - Isolate DNA from the most difficult plant types, including strawberry leaf, cotton leaf, cotton seeds and pine needles
- Inhibitor Removal Technology - Removes polyphenolics, polysaccharides and other PCR inhibitors
The DNeasy PowerPlant Pro HTP 96 Kit is designed for high-throughput purification of genomic DNA from plant cells, tissues and seeds. The optimized protocol replaces time-consuming DNA isolation procedures such as CTAB, phenol, or chloroform extraction for recovery of high quality DNA from the toughest sample types, including strawberry leaf, cotton leaf, cotton seeds, and pine needles. Patented Inhibitor Removal Technology is used for removal of PCR inhibitors, resulting in pure DNA that is ready to use in downstream applications including PCR, QPCR, Sanger sequencing and NGS. In addition, a unique Phenolic Separation Solution (PSS) is included as an optional step for samples high in polyphenolic compounds, such as pine and grape leaf. PSS breaks the bond between DNA and phenolics, preventing loss of DNA during the IRT step and increasing DNA yield. Following an inhibitor removal step, genomic DNA is captured on a 96 well silica membrane in a spin filter format. DNA is then washed and eluted from the spin filter plate. DNA is ready to use in PCR, qPCR or next generation sequencing. The kit can be used in combination with bead beating or another lysis method. Metal Beads are recommended for bead beating.
Formerly known as the PowerPlant Pro-htp 96 Well DNA Isolation Kit
Format Silica Spin Filter Plates Method Bead Beating Sample Size Up to 50 mg Binding Capacity Up to 20 µg per prep Throughput 96-384 samples Time Process 384 samples in less than one day Storage RNase A should be stored at 4°C
The other kit reagents and components should be stored at room temperature (15-30°C)
Bead Type (not included with kit) Metal Beads are recommended for bead beating and can be purchased separately, catalog # 13117-500 Sample Types Processed Plants and seeds Equipment Required TissueLyser II and Plate Adapter Set
96 well plate centrifuge
Catalog # 13496-4 Old Name PowerPlant Pro-htp 96 Well DNA Isolation Kit
Figure 1. Isolation of high quality genomic DNA from plant samples using the DNeasy PowerPlant Pro HTP 96 Kit. DNA isolated from grass (2 ul), strawberry leaf (15 ul), grape leaf (15 ul) and pine needles (1 ul) was examined on a 1% TAE agarose gel. Figure 2. The DNeasy PowerPlant Pro HTP 96 Kit provides greater yields of high molecular weight genomic DNA. DNA was isolated from cotton seed using the DNeasy PowerPlant Pro HTP 96 Kit and plant DNA isolation kits from three other suppliers. All isolations were performed according to the manufacturer’s protocol. DNA (2% of elution volume) was examined on a 1% TAE agarose gel. Figure 3. Successful amplification of DNA isolated using the DNeasy PowerPlant Pro HTP 96 Kit. QPCR was performed using primers for RuBisCo on 1 ng of DNA isolated from pine needles (A) and strawberry leaf (B). Undiluted DNA and samples diluted 1:10 both fell within the standard curve (purple lines), and the difference between the diluted and undiluted samples was approximately 3 cycles.