WeLLevator Pipette Guide

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WeLLevator Pipette Guide

Facilitates consistent and fatigue-free extraction of liquids from 96 well plates when using a multichannel pipette and enables the manual use of MagAttract kits when paired with a PowerMag Magnet

Size/Component Catalog #
1 unit 11970
Product Description

Details

  • Consistent results - Manually extract the maximum volume of supernatant from 96 well plates without concern about disturbing a pellet, and ensure uniform handling of samples in every well

  • Fatigue-free pipetting - Rest your multichannel pipette on top of the WeLLevator® Pipette Guide for effortless pipetting

  • Increase your throughput without a robot - Convert our MagAttract magnetic bead kits to manual kits with just a WeLLevator and a PowerMag Magnet

Manual extraction of liquids from 96 well plates using multichannel pipettes can be mechanically awkward and tedious. The sheer number of wells and the opacity of the plate make it difficult to avoid pelleted materials and to achieve well-to-well consistency. The WeLLevator Pipette Guide (11970) is designed to facilitate consistent and fatigue-free extraction of liquids from SBS formatted 96 well plates when using a multichannel pipette. The WeLLevator Pipette Guide can be used with either 8 or 12 channel pipettes. 

SDS/Protocol

SDS/Protocol

Protocol

Data

Data

Consistent results using a WeLLevator Pipette Guide with the MagAttract PowerClean DNA Kit
       
A   Figure 1. The MagAttract PowerClean DNA Kit effectively removes UV-absorbing contaminants from isolated DNA using either a KingFisher Duo or a WeLLevator Pipette Guide. (A) DNA isolated from 0.25 grams of garden soil without any upfront inhibitor removal (i.e., Inhibitor Removal Technology, IRT) is highly contaminated with UV-absorbing components that mask the true concentration of DNA found in the sample (“Garden soil, no IRT”). 100 µL technical replicates of this contaminated material was processed through the MagAttract PowerClean DNA Kit on a KingFisher Duo or the MO BIO WeLLevator Pipette Guide equipped with a PowerMag Magnetic Separator to obtain highly purified, PCR-inhibitor depleted DNA (“PowerMag DNA Clean-Up”). Note: Due to evaporative loss of water from the elution buffer during the KingFisher protocol, samples processed on the robot are usually more concentrated than those processed by hand on the WeLLevator. This is reflected here in the NanoDrop 260 nm absorbance reading.(B) Taking the final elution volumes into account, calculation of the % DNA recovery reveals that the WeLLevator-assisted magnetic bead protocol delivers excellent DNA yields and quality via spectrophotometric analysis. (C) DNA samples described in (B) were examined via 1.2% agarose gel, demonstrating that equivalent yields and high quality DNA are obtained using either a WeLLevator or KingFisher Duo protocol.       
     
B  
     
C