RNeasy PowerPlant Kit

RNeasy PowerPlant Kit
Isolate total RNA from plant and seed samples, including those high in polyphenols and polysaccharides.
Size/Component Catalog #
50 preps 13500-50
Product Description

Details

  • Optimized for tough samples - Isolate RNA from the most difficult plant types, including strawberry leaf, cotton leaf, cotton seeds and pine needles
  • Inhibitor Removal Technology- Removes 100% of polyphenolics, polysaccharides and other PCR inhibitors
  • Rapid protocol - Enables isolation of RNA in 30 minutes without the use of harsh chemicals

Isolate pure, high quality RNA from the toughest plant types. The RNeasy PowerPlant Kit is designed for fast and easy purification of total RNA from plant cells, tissues and seeds. The bead beating technology used in this kit replaces potentially damaging RNA isolation procedures such as CTAB and TRIZOL extraction for recovery of high quality RNA from the toughest sample types, including strawberry leaf, cotton leaf, cotton seeds, and pine needles. Patented Inhibitor Removal Technology is used for removal of PCR inhibitors from plant extracts during the isolation process, resulting in pure RNA that is ready to use in RT-qPCR and RNA-seq. In addition, a unique Phenolic Separation Solution (PSS) is included as an optional step for samples high in polyphenolic compounds, such as pine and grape leaf. PSS breaks the bond between RNA and phenolics, preventing loss of RNA during the IRT step and increasing RNA yield.

miRNA can also be isolated using this kit. The RNeasy PowerPlant Kit may be used with a vortex (for soft leaf tissue) or high velocity bead beater, such as the PowerLyzer 24 Homogenizer. The PowerLyzer 24 is suitable for rapid homogenization of plant materials including stems, roots, seeds or difficult leaf tissue.

Formerly known as the PowerPlant RNA Isolation Kit.

Specs

Specs

Format Silica Spin Filter Tubes
Method Bead Beating
Sample Size Up to 50 mg of plant material
Binding Capacity Up to 40 µg per filter
Throughput 1-24 samples
Time 30 minutes
Storage
Store at room temperature (15-30°C)
Bead Type 2.8 mm ceramic beads
Sample Types Processed Plants and seeds
Equipment Required Vortex and Vortex Adapter (13000-V1-24) or PowerLyzer 24 Homogenizer
Catalog # 13500-50, 13500-S
Old Name PowerPlant RNA Isolation Kit
SDS/Protocol
Data

Data

The PowerPlant® RNA isolation kit provides higher yields of intact RNA from tough samples.   Successful amplification of RNA isolated using the PowerPlant® RNA Isolation Kit.
     
Figure 1. The RNeasy PowerPlant Kit provides higher yields of intact RNA from tough samples. Total RNA was isolated using the RNeasy PowerPlant Kit and plant RNA isolation kits from two other suppliers. All isolations were performed according to the manufacturer’s protocol. RNA samples from cotton leaf (8 µl) and seed (2 µl) were examined on a 1% TAE agarose gel.   Figure 2. Successful amplification of RNA isolated using the RNeasy PowerPlant Kit. RT-qPCR was performed using primers for RuBisCo on 1 ng of RNA isolated from cotton leaf (A) and seed (B). All samples fell within the standard curve (purple lines).