MagAttract PowerWater DNA/RNA Kit

MagAttract PowerWater DNA/RNA Kit
Automated isolation of nucleic acids from filtered air and water samples.
Size/Component Catalog #
4 x 96 Preps 27800-4-EP
Product Description

Details

  • Inhibitor Removal Technology - Enables kit to excel with the most challenging air and turbid water samples
  • ClearMag Technology - Proprietary magnetic particle technology captures nucleic acids without binding organic inhibitors, facilitating isolation of high yields of pure DNA and RNA
  • Optimized bead mix - 5 ml bead tubes containing a novel bead mix have been tested and validated to work with the most common filter membrane types
  • Hands-free purification - Optimized for use with automated liquid handling systems*

The MagAttract PowerWater DNA/RNA Kit is a magnetic bead based nucleic acid isolation kit that can be used for automated isolation of microbial DNA and RNA from any liquid or air sample that is concentrated on a filter membrane. Filter membranes composed of a variety of materials can be processed with this kit (see table in specifications). Filtered samples containing inhibitors such as salts, metals, humic substances and other organic materials are easily processed using patented Inhibitor Removal Technology (IRT) to remove PCR inhibitors released during the extraction process. A novel, proprietary magnetic bead system is used for the isolation of nucleic acids without the binding of residual contaminants, for inhibitor-free DNA and RNA that is ready to use in the most demanding downstream applications including PCR, qPCR, RT-qPCR, RNA-seq, Sanger sequencing and NGS.

For maximum sample processing, this kit requires the use of a specialized plate shaker in order to facilitate the bead beating process in 5 ml PowerWater Bead Tubes. We recommend the TissueLyser II and the 5 ml Tube Adapters (MO BIO Catalog# 11980), which can accommodate up to 32 PowerWater Bead Tubes. Also available is the 5 ml Tube Centrifuge Block (MO BIO Catalog # 11981) which accommodates 32 PowerWater Bead Tubes for easy centrifugation in standard 96 well centrifuge buckets.

*The MagAttract PowerWater DNA/RNA Kit is designed for use with automated liquid handling systems, such as the Tecan, Hamilton, Beckman and Eppendorf systems. A protocol is available for the Eppendorf epMotion. For other automated liquid handling systems, please contact technical@mobio.com for assistance prior to purcasing the kit.

Formerly known as the PowerMag Air & Water DNA/RNA Isolation Kit.

Specs

Specs

Format ClearMag Technology
Method Bead Beating
Throughput 96 well plate
Time 45 minutes
Storage Store at room temperature (15-30°C)
Bead Type 0.15 mm, 0.7 mm garnet mix
Sample Types Processed Ocean water, fresh water, brackish water, ground water, waste water, air samples
Equipment Required TissueLyser II and 5 ml Tube Adapter Set
Centrifuge capable of handling two 96 well blocks
Automated liquid handling system
Old Name PowerMag Air & Water DNA/RNA Isolation Kit
SDS/Protocol
Data

Data

High quality DNA and RNA from ocean water samples
    
High quality DNA and RNA from ocean water samples       
Membrane Type DNA
(ng/µl)
RNA
 (ng/µl)
A260/280
  MCE, 0.45 µm  9.4 8.8 1.72
  PES, 0.22 µm  10.6 19.1 1.77
  PC, 0.8 µm  8.1 10.8 1.77
 
    
Figure 1. 500 ml of coastal ocean water was filtred through each 47 µm filter membrane type in duplicate. DNA and RNA yields were determined using a Qubit™ Fluorometer. Purity ratios were calculated using a NanoDrop™ Spectrophotometer. All calculated values were averaged between duplicates. MCE: mixed cellulose ester; PES: polyethersulfone; PC: polycarbonate.
  

 

Reliable Nucleic Acid Purification from Diverse Water Samples
     
Reliable Nucleic Acid Purification from Diverse Water Samples   Water samples were filtered through mixed cellulose ester (MCE) membranes in duplicate until the flow stopped.
     
Sample #    Sample TypeDNA
(ng/µl)
RNA 
(ng/µl)
A260/280
1     Brackish Lagoon 41.7 29.0 1.81
2     Fresh Water Lake 8.9 13.0 1.82
3     Ocean Water 3.2 3.5 1.91
 
   
Figure 2. Water samples were filtered through mixed cellulose ester (MCE) membranes in duplicate until the flow stopped.  DNA and RNA yields were determined using a Qubit™ Fluorometer.  Purity ratios were calculated using a NanoDrop™ Spectrophotometer.  All calculated values were averaged between duplicates.  The Molzyme 16S Complete qPCR assay was used for general microbial detection.   

 

Efficient Nucleic Acid Extraction and Purification from Air Filter Membranes
     
200 µl of an overnight B. subtilis culture was spotted onto each 25 mm filter membrane.  
Membrane TypeDNA (ng/µl)  RNA (ng/µl)A260/280
  MCE, 0.45 µm 4.6 16.7 1.94
  PES, 0.22 µm 4.7  18.7  1.98
  PC, 0.8 µm 2.4  6.5  2.04
  Culture Control 2.4  15.6 1.86
     
Figure 3. 200 µl of an overnight B. subtilis culture was spotted onto each 25 mm filter membrane.  The same amount of culture was stored as a pellet at 4°C (control). All samples were incubated overnight. DNA and RNA yields were determined using a Qubit™ Fluorometer.  Purity ratios were calculated using a NanoDrop™ Spectrophotometer.  All calculated values were averaged between duplicates.