AllPrep PowerViral DNA/RNA Kit

AllPrep PowerViral DNA/RNA Kit
Isolate viral or bacterial total nucleic acids from waste water and stool samples.
Size/Component Catalog #
50 preps, including Glass Bead Tubes 28000-50
Product Description

Details

  • Optimized for challenging samples - Isolate viral or bacterial DNA and RNA from waste water, stool, biosolids and gut material
  • Inhibitor Removal Technology- Eliminates inhibitory substances, including polysaccharides, lipids and heme for nucleic acids that are ready to use in PCR, qPCR, RT-qPCR, RNA-Seq, Sanger sequencing or NGS.
  • Optional bead beating protoocol- Available with Glass Bead Tubes for optimal lysis of solid samples and bacterial cells and without bead tubes for isolation of viral nucleic acids from liquid samples

The Allprep PowerViral DNA/RNA Kit is designed for fast and easy purification of viral and bacterial total nucleic acids from samples high in PCR inhibitors; including waste water, stool, biosolids, and gut material. Patented Inhibitor Removal Technology (IRT) ensures complete removal of the inhibitory substances often contained in these materials, such as undigested plant material in the gut or heme compounds from lysed red blood cells, abundant in stool. The result is DNA and RNA  from virus and bacteria that is ready to use in the most demanding downstream applications. Nucleic acids are eluted in RNase-Free Water and ready for PCR, cDNA synthesis, RT-qPCR, RNA-seq, Sanger sequencing or NGS.  

For stool samples or samples with a solid matrix, lysis is achieved using 0.1 mm Glass Bead Tubes in combination with a strong chemical lysis buffer that ensures efficient extraction of tough microorganisms in the bead beating step. For virus extraction from water samples and samples that do not require dispersion, the bead beating step may be skipped and virus extracted through chemical lysis only.

If lysis with Glass Bead Tubes is required, purchase Catalog # 28000-BUNDLE, which contains the Allprep PowerViral DNA/RNA Kit plus 0.1mm Glass Bead Tubes. Otherwise, purchase Catalog # 28000-50 which is the Allprep PowerViral DNA/RNA Kit alone.  If additional DNase treatment is required, we recommend the DNase Max Kit.

The Allprep PowerViral DNA/RNA Kit (catalog # 28000-50) has been updated to include 0.1 mm glass bead tubes. If you previously ordered 28000-BUNDLE, you can now order 28000-50. For cell lysis, bead beating is optional. It should be used when lysis of microbial cells is desired or if the starting sample contains solid material that needs dispersing. 

Formerly known as the PowerViral Environmental RNA/DNA Isolation Kit.

Specs

Specs

Format Silica Spin Filter Tubes
Method Chemical lysis and/or bead beating
Sample Size 200 µl or 0.25 g
Binding Capacity Up to 40 µg per spin filter
Throughput 1-24 samples
Time 45 minutes
Storage Store at room temperature (15-30°C)
Bead Type 0.1 mm glass (optional)
Sample Types Processed Waste water, stool, biosolids, and gut material
Equipment Required Vortex
Vortex Adapter (13000-V1-24) (optional for bead beating)
Old Name PowerViral Environmental RNA/DNA Isolation Kit
SDS/Protocol
Publications

Publications

Formerly known as the PowerViral Environmental RNA/DNA Isolation Kit.