DNeasy PowerSoil Pro Kit
- Efficiently lyse bacteria and fungus in all soil types, including compost, clay and top soil
- Isolate 20-fold more DNA per run
- Recover ready-to-use, inhibitor-free DNA in downstream NGS applications
- Get unbiased results with high sequencing diversity
- Automate with the QIAcube
Both the original DNeasy PowerSoil and new DNeasy PowerSoil Pro Kits isolate high-quality DNA from challenging soil types. However, the DNeasy PowerSoil Pro isolates of up to 8X higher yields of DNA compared to the first generation DNeasy PowerSoil kit and competitors in all soil types tested, utilizing a novel bead tube and improved lysis technology (Figures 1 and 2). DNA isolated using DNeasy PowerSoil Pro identified more bacteria (OTUs) in soil samples when compared with the DNeasy® PowerSoil® kit and two competitors (Figure 3).
The soil or stool samples are lysed via chemical and mechanical homogenization. Lysis buffer is added to a mixed zirconium bead tube containing the sample. Bead beating can be carried out using a standard benchtop vortex with bead tube adapter or the high-powered TissueLyzer. Crude lysate is then subjected to inhibitor removal for clean-up. Following the clean-up, purified lysate is mixed with an equal volume of DNA binding solution and passed through a silica spin filter membrane. The membrane is washed with a two-step washing regime. Silica bound DNA is then eluted using a 10mM tris elution buffer. DNA applications include PCR, NGS, and enzymatic digestion assays.