NoviPure Microbial Protein Kit
- Powerful lysis - Optimized bead beating method enables efficient lysis and solubilization of total proteins from fungi, gram-negative and gram-positive bacteria
- Easy and fast protein extraction - Novel spin filter-based method enables protein extraction in just 22 minutes without the need for precipitation
- Accurate results - Protein can be used for mass spectrometry, 1D and 2D gel electrophoresis and other proteomics applications
This novel protein isolation method begins with mechanical lysis of cultured microbial cells using a mixed glass bead tube. Proteins released during homogenization are denatured and solubilized by a strong chaotropic agent. An optimized two-step binding process is then used to immobilize all solubilized proteins onto a silica spin filter. In the first step, a salt solution is added to the microbial lysate and passed over a silica spin filter to bind most proteins. In the second step, the flow through is adjusted with a second salt buffer and passed through the same spin filter. This second salt buffer promotes low molecular weight protein association with the silica spin filter. Immobilized proteins are then washed and finally eluted.