DNeasy UltraClean Microbial Kit

DNeasy UltraClean Microbial Kit
For isolation of high quality DNA from microbial cultures.
Size/Component Catalog #
50 preps 12224-50
250 preps 12224-250
Product Description

Details

  • Rapid protocol - Isolate DNA from microbial cultures in just 20 minutes
  • Isolate pure, high-quality DNA - Ready to use in PCR, qPCR, restriction digests, Sanger sequencing and NGS
  • Optimized lysis method - Mechanical lysis via bead-based homogenization enables isolation of DNA from yeast, fungi, Gram-negative and Gram-positive bacteria

The DNeasy UltraClean Microbial Kit is designed to isolate high quality genomic DNA from 1.8 ml of microbial culture. A variety of microorganisms including Gram-negative and Gram-positive bacteria, yeast, and bacterial and fungal spores have been tested successfully with this kit. The microbial cells are added to a bead beating tube containing beads, bead solution and lysis solution. The principal is to lyse the microorganisms by a combination of heat, detergent, and mechanical force using an optimized bead beating tube. The cells are lysed using our specially designed Vortex Adapter on a Vortex Genie 2 Vortex. The released DNA is then bound to a silica spin filter, washed and the DNA is recovered in certified DNA-free Tris buffer. 

For isolation of microbial DNA using a bead-based homogenizer, try our DNeasy PowerLyzer UltraClean Microbial Kit.

Formerly known as the UltraClean Microbial DNA Isolation Kit.

Specs

Specs

Format Silica Spin Filter Tubes
Method Bead Beating
Sample Size 1.8 ml
Binding Capacity Up to 20 µg per filter
Throughput 1-24 samples
Time 30 minutes
Storage Store at room temperature (15-30°C)
Bead Type 0.15 mm garnet
Sample Types Processed Cultured gram-negative bacteria, gram-positive bacteria, yeast, fungi
Equipment Required Vortex and Vortex Adapter (13000-V1-24)
Catalog # 12224-50, 12224-250, 12224-S
Old Name UltraClean Microbial DNA Isolation Kit

 

SDS/Protocol

SDS/Protocol

Protocol

SDS

Data

Data

High quality DNA purified from a variety of microorganisms 
Lane 1: 1 kb ladder
Lane 2: E. coli
Lane 3: P. aeruginosa
Lane 4: S. aureus
Lane 5: M. luteus
Lane 6: B. subtilis
Lane 7: B. pumulis
Lane 8E. faecalis
Lane 9L. casei
Lane 10: 1 kb ladder
Lane 11A. niger
Lane 12C. albicans
Lane 13S. cerevisiae
Lane 14S. cerevisiae
  Figure 1. Analysis displayed on 1.2% TAE agarose gel. Lower yields obtained with A. niger (11) and C. albicans (12) due to lower cell densities in starting culture.
Publications

Publications

TESTIMONIALS
Write a Testimonial
Works for whole genome sequencing

Review by E.M. / (Posted on 11/12/2015)

<p style="text-align: justify;">I received the sample and used it to prep gDNA of <em>B. subtilis</em>, getting great yield and quality DNA on the first try. I used the samples for whole genome sequencing and received perfect quality scores from start to finish. So, all in all I am very happy with the product and will make sure to mention it in the methods of the eventual paper from this research. It might be a while before we order as we only very rarely require gDNA, but I will recommended it to my lab for the next time. We had been doing messy phenol/chloroform/iso-amyl alcohol extractions before now!</p>

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