ATP-Free Certification

ATP-Free Certification
This test is used to certify a variety of products to be free of contaminating ATP molecules which can interfere with several types of enzymatic reactions and also further indicate the presence of any biological activity.
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Service Description

Details

  • Experienced - MO BIO has over 22 years of experience in providing ATP-free certifications to many major medical device companies and suppliers of plastic consumables
  • Qualified - All MO BIO Services technicians receive extensive training and are qualified to test raw materials or end products for the presence of ATP
  • Certified - MO BIO is ISO 9001:2008 certified for providing 3rd party independent laboratory testing services for the life science industry

Adenosine triphosphate (ATP) is a nucleoside triphosphate used in cells as a coenzyme and is is the universal molecule of energy storage that is produced by all living things. Since ATP is a constituent of all living cells, including bacteria and fungi, it is a reliable indicator of microbial contamination. The presence and amount of ATP is an indication of the level of purity of that product. During the manufacturing process, human handling of and exposure to manufacturing materials and machinery creates opportunities for product contamination to occur. Common sources of ATP contamination include finger tips, saliva, hair follicles, nasal cavity, ear canal, human interaction, food residues, water, soil particles and animals.

Certifying products ATP-free has become a common practice among general laboratory plastics manufacturers as well as medical device and pharmaceutical companies. Testing your product lines for this common contaminant will add integrity to your products and will enable a wider range of customers to utilize your products for their experiments and applications.

Methods

Methods

ATP Test Method

A luciferase-based assay is used to detect the level of ATP in a sample of product. Luciferase is the enzyme found in fireflies that causes them to emit light. The luciferase enzyme catalyzes an ATP dependent oxidation of the luciferin substrate (shown in the reaction below).

ATP + D Luciferin + O2 --> Luciferase --> AMP + oxyluciferin + CO + PPi + light

The product samples are extracted in an extract liquid which is then combined with the luciferase enzyme. The light that is emitted during this reaction is measured by a device called a luminometer in relative light units (RLUs) and is proportional to the amount of ATP present. An ATP standard positive and a negative control are tested along with the product extract.

From the positive control a baseline cut-off value is calculated, if the product extract results in an ATP level that exceeds this value then the product is contaminated with ATP. If a product tests positive for ATP, it may be contaminated with microorganisms or other cellular debris. Further biological testing may be needed to verify the amount and type of contaminant that is present.

Test Sensitivity: 10-13 mg/µl

Contact Services

Contact Services

Our Services team has over 23 years of experience in providing services that keep your products competitive and in demand. Our customers include many of the top plastics and medical device suppliers in the industry. Our services specialists can also work with you to develop a custom testing schedule and/or custom tests that fit the specific needs of your products. We are located in Carlsbad, CA, USA.

CONTACT SERVICES

800-606-6246
   
service@mobio.com