DNA-Free Certification

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DNA-Free Certification
This test certifies any of a wide variety of products to be free of certain types of DNA and common contaminants and inhibitors that interfere with the PCR process.
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Service Description

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MO BIO offers two test methods for DNA-Free Certification: Test Method 1 (Test Sensitivity 30 pg) Test samples are extracted and a portion of extract is added to a multiplex PCR reaction containing primers specific for human and mouse genomic DNA. These tubes receive no template DNA so any amplification in these tubes will indicate the presence of contaminating DNA. A negative control reaction is done with DNA-Free water as a reference. In another set of reactions that test for PCR inhibition, DNA is added to tubes containing the multiplex PCR reaction and product extract. A positive control reaction is performed with DNA-Free water as a reference. Template human and mouse DNA are then added to the product extract and positive control tubes. After 40 cycles of amplification the reactions are evaluated by agarose gel electrophoresis. The first set of reactions without DNA should produce only primer bands. No DNA should be amplified unless there is either human or mouse DNA contamination. The second set of reactions should produce three bands. One band representing the primers. Two higher molecular weight bands should appear which represent a human DNA band at 270 bp, and a mouse DNA band at 420 bp. Passing test criteria require no DNA bands in negative reactions and two DNA bands in all positive reactions. Products are tested for the presence of human and mouse DNA contamination using species specific PCR primers
Methods

Methods

MO BIO offers two test methods for DNA-Free Certification: Test Method 1 (Test Sensitivity 30 pg) Test samples are extracted and a portion of extract is added to a multiplex PCR reaction containing primers specific for human and mouse genomic DNA. These tubes receive no template DNA so any amplification in these tubes will indicate the presence of contaminating DNA. A negative control reaction is done with DNA-Free water as a reference. In another set of reactions that test for PCR inhibition, DNA is added to tubes containing the multiplex PCR reaction and product extract. A positive control reaction is performed with DNA-Free water as a reference. Template human and mouse DNA are then added to the product extract and positive control tubes. After 40 cycles of amplification the reactions are evaluated by agarose gel electrophoresis. The first set of reactions without DNA should produce only primer bands. No DNA should be amplified unless there is either human or mouse DNA contamination. The second set of reactions should produce three bands. One band representing the primers. Two higher molecular weight bands should appear which represent a human DNA band at 270 bp, and a mouse DNA band at 420 bp. Passing test criteria require no DNA bands in negative reactions and two DNA bands in all positive reactions. Products are tested for the presence of human and mouse DNA contamination using species specific PCR primers
Submission Form

Submission Form

Submission Form
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