RNase Test Method
All testing apparatus are treated with Diethyl Pyrocarbonate (DEPC) to inhibit possible RNase contamination of the experiment from outside sources. Test samples of the product are extracted and a portion of extract is incubated with an RNA standard (6 kb RNA) in a buffered solution with both sodium and magnesium ions. Negative and Positive controls are run with the test sample extract to validate the experimental procedure and technique. An unexposed RNA standard is included to represent the negative control. An RNA standard exposed to RNase is also included to represent the positive control.
All samples are incubated 1 hour at 37oC. After the incubation period, the samples and controls are evaluated by agarose gel electrophoresis and a picture for the Certificate of Analysis and final report is taken. The test samples and the negative control should show no degradation. The positive control should be a very blurry, quick-running band, indicating the effect of RNase contamination on the RNA standard pool that is used for all the samples.