DNeasy PowerClean Pro Cleanup Kit
- Efficient secondary purification - Fast and easy 7 minute protocol to clean up your problematic DNA
- Removes challenging impurities - Purifies nucleic acids containing humic substances, polyphenolics, polysaccharides and other PCR inhibitors
- Successful amplification - High purity nucleic acids for use in PCR, qPCR and next generation sequencing
Clean-up problematic DNA in half the time of the original DNeasy PowerClean Cleanup Kit. The DNeasy PowerClean Pro Cleanup Kit utilizes our Inhibitor Removal Technology® to provide researchers with a novel and proprietary method for cleaning up previously isolated genomic DNA. This kit is a significantly streamlined improvement over the original PowerClean® DNA Clean-Up Kit, with a 7 minute protocol that includes fewer steps.
Starting DNA may be amber to brown in appearance; an indicator of PCR inhibiting substances, particularly humics and polyphenols. Even samples that appear colorless may contain PCR inhibitors which can be cleaned up with this kit. The DNeasy PowerClean Pro Cleanup Kit will remove this brown color as well as any PCR inhibiting substances, such as humic acids, heme, polysaccharides, polyphenols, fulvic acids, lipids and dyes. The resulting high purity DNA is ready to use in the most demanding downstream applications, including PCR, qPCR and next generation sequencing.
This kit was validated with DNA isolated from a variety of problematic soils and also with DNA samples spiked with commercial humic acids. However, it performs well on DNA isolated from virtually any sample source. Archived or previously isolated DNA samples are purified when combined with our proprietary DNA Clean-Up reagents., and inhibitors are selectively removed from the DNA solution. All DNA, including total genomic DNA, is captured on a silica membrane in a spin column format. DNA is then washed and eluted from the membrane. Percentage recovery varies depending on the level of inhibitors in the DNA that may be influencing the DNA yield measurement. Purified DNA is ready for PCR analysis and other downstream applications. We recommend quantifying DNA via a PicoGreen assay or running DNA on an agarose gel to determine recovery yield.
Formerly known as the PowerClean Pro DNA Clean-Up Kit.
Cat No./ID: 12997-50
DNeasy PowerClean Pro Cleanup Kit (50)
Remove PCR inhibitors from purified DNA in just 7 minutes.
The DNeasy PowerClean Pro Cleanup Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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