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NoviPure Microbial Protein Kit

Isolate total cellular proteins from microbial cultures.
  • Powerful lysis - Optimized bead beating method enables efficient lysis and solubilization of total proteins from fungi, gram-negative and gram-positive bacteria
  • Easy and fast protein extraction - Novel spin filter-based method enables protein extraction in just 22 minutes without the need for precipitation
  • Accurate results - Protein can be used for mass spectrometry, 1D and 2D gel electrophoresis and other proteomics applications
The NoviPure Microbial Protein Kit is designed to isolate total cellular proteins from microbial cultures in a quick and user-friendly spin filter format. An optimized bead beating method enables efficient lysis and solubilization of total proteins from a large diversity of microbial species including fungi, gram-negative and gram-positive bacteria. The use of silica spin filters to achieve reversible immobilization of proteins — a patent-pending technical advance in protein extraction — greatly simplifies the isolation process by removing traditional cumbersome and bias-inducing precipitation steps. Isolated proteins are suitable for many downstream applications such as 1D and 2D SDS PAGE as well as mass spectrometry.

Cat No./ID: 47044
NoviPure Microbial Protein Kit (50)
Isolate total cellular proteins from microbial cultures.
$383.00
The NoviPure Microbial Protein Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Procedure

This novel protein isolation method begins with mechanical lysis of cultured microbial cells using a mixed glass bead tube. Proteins released during homogenization are denatured and solubilized by a strong chaotropic agent. An optimized two-step binding process is then used to immobilize all solubilized proteins onto a silica spin filter. In the first step, a salt solution is added to the microbial lysate and passed over a silica spin filter to bind most proteins. In the second step, the flow through is adjusted with a second salt buffer and passed through the same spin filter. This second salt buffer promotes low molecular weight protein association with the silica spin filter. Immobilized proteins are then washed and finally eluted.