QIAGEN Microbiome Webinars
Semi-automated low-throughput workflow for microbial analyses of human stool
Tue, Feb 21, 2017 10:00 AM - 11:00 AM PST
The gut microbiota composition changes dramatically throughout ageing and disease. A healthy gut microbiota is typically characterized by large bacterial taxonomic diversity and functional capacity, whereas frailty and ageing are associated with loss of diversity and expansion of more pathogenic bacterial species. However, in order to accurately profile changes in microbial communities, the reproducible isolation of high quality DNA is an important step. Automation allows for convenient, reliable and reproducible isolation of high quality DNA, which can be used directly for downstream sequencing applications. In this webinar, we will discuss the development of a semi-automated workflow to profile the gut microbiota of young and old individuals and identify changes in bacterial composition and function that occur with age. This workflow will help to simplify and streamline the DNA extraction process for samples with high inhibitor content and subsequent microbial community analyses.
Speaker: Patrick Smith, Ph.D., Scientist R&D, QIAGEN
Microbiome analysis – from complex samples to insight with confidence
Whether you’re knee deep in microbial research or wondering how to design your first study, the best chance of achieving reliable results and advancing your microbiome research is choosing the right tools for your workflow. Now that MO BIO is part of QIAGEN, we can provide everything you need for your microbiome studies beginning with DNA/RNA isolation, continuing with NGS and ending with data analysis. MO BIO’s optimized bead beating technology and patented Inhibitor Removal Technology® ensure high quality, inhibitor-free DNA that is ready to use in downstream amplification or NGS library preparation.
QIAGEN Microbiome is offering a new webinar series to help you advance your microbiome research
Part 1: Nucleic acid isolation from PCR inhibitor-rich sample types
Presenter: Eddie Adams, PhD – Director of R&D, MO BIO Laboratories
In this webinar, we focus on nucleic acid extraction tools developed by MO BIO Laboratories that facilitate accurate non-biased community analysis and eliminate common amplification problems via the depletion of endogenous polymerase inhibitors using our patented Inhibitor Removal Technology.
Part 2: QIAseq technologies for metagenomics and microbiome NGS library prep
Presenter: Jennifer Fostel, PhD – Senior Global Product Manager, QIAGEN
In this webinar, learn about the innovative technologies that form the basis of QIAGEN’s portfolio of QIAseq library prep solutions for metagenomics and microbiome sequencing. Whether your research starts from single microbial cells, 16s rRNA PCR amplicons, or gDNA for whole genome analysis, QIAseq technologies offer tips and tricks for capturing the genomic diversity of your samples in the most unbiased, streamlined way possible.
Part 3: Microbiome profiling with the Microbial Genomics Pro Suite
Presenters: Arne Materna, PhD – Director of Microbial Genomics, QIAGEN and Andreas Sand Pedersen, PhD – Senior Research Bioinformatician, QIAGEN
In this webinar, we introduce the scientist-friendly Microbial Genomics Pro Suite offering workflows optimized for microbiome profiling, microbial typing and outbreak analysis. The workflows and tools for microbial genomics introduced with this software package further extend the comprehensive set of genomics, transcriptomics and epigenomics analysis solutions that researchers know from CLC Genomics Workbench.
The webinar focuses on how users can, with a few simple steps, analyze 16S rRNA data to obtain and compare taxonomic profiles of microbial communities. You will also learn how to assemble and annotate metagenomes to generate functional profiles, and how to carry out statistical comparisons of relative abundance between sample groups in the context of experiment-relevant metadata.
- 1. Sender, R., Fuchs, S., Milo, R. (2016) Are we really vastly outnumbered? Revisiting the ratio of bacterial to host cells in humans. Cell 164, 3. Link
- 2. Kroes, I., Lepp, P., Relamn, D. (1999) Bacterial diversity within the human subgingival crevice. PNAS 96, 25. Link
Webinar library coming soon!